What are post-translational modifications?
Protein post-translational modifications (PTMs) increase the functional diversity of the proteome by the covalent addition of functional groups or proteins, proteolytic cleavage of regulatory subunits, or degradation of entire proteins [1]. PTMs increase complexity from the genome to the proteome level. They are chemical modifications that play a key role in functional proteomics because they regulate activity, localization, and interaction with other cellular molecules such as proteins, nucleic acids, lipids and cofactors [1].
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CLN3 Phosphorylation
NetPhos was used to create tables of bona fide phosphorylation sites on a given protein. Below are the comparisons between the human and fly CLN3 protein sequences. The bars above the pink horizontal line indicate a high confidence level of being a true phosphorylation site.
Conclusion
The conserved phosphorylation sites between the Homo sapien and Drosophila melanogaster CLN3 protein give insight to where scientists should focus their studies. Specifically, those above the pink threshold should be of the central focus, because they are likely true phosphorylation sites. Should an amino acid sequence be phosphorylated in a conserved region, it is likely that both the Homo sapien and the Drosophila melanogaster will express the same phenotype.
References
Background: http://www.interactive-biology.com/6711/the-basics-of-protein-structure-and-function/
Figure 1. :https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-post-translational-modification.html
[1] https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-post-translational-modification.html
Figure 1. :https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-post-translational-modification.html
[1] https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-post-translational-modification.html
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