What are protein interaction networks?
Protein-protein interaction networks (PPIN) are representations of the physical connections between proteins in the cell. These connections are specific, occur between defined binding regions in the proteins, and have a specific function [1]. They allow the formation of protein complexes and mediate post-translational protein modifications. Disruption of protein-protein interactions may result in disruption of the cell component or process to which they contribute, compromising the cell viability or even leading to cell death [2].
CLN3 Interactions Networks
The protein interaction networks of CLN3 in both humans and fruit flies are shown below. While the majority of the proteins are involved in lipid or protein binding, both highlighted regions are interestingly involved in RNA binding. All of the proteins that interact with CLN3 have a transmembrane domain suggesting that they all are found within the same region of the CLN3 protein; presumably on the neuronal lysosome.
Human |
Fruit Fly |
Conclusion
The above protein interaction networks were created via the STRING database. None of the proteins in the interaction network had unexpected functions, with the exception of RNA binding proteins (highlighted). There was also a great amount of membrane fusion proteins, meaning it is likely CLN3 does not retain this function.
References
[1] https://www.ebi.ac.uk/training/online/course/network-analysis-protein-interaction-data-introduction/protein-protein-interaction-networks
[2] Vazquez A. Protein Interaction Networks. In: Alzate O, editor. Neuroproteomics. Boca Raton (FL): CRC Press/Taylor & Francis; 2010. Chapter 8. Available from: https://www.ncbi.nlm.nih.gov/books/NBK56024/
[2] Vazquez A. Protein Interaction Networks. In: Alzate O, editor. Neuroproteomics. Boca Raton (FL): CRC Press/Taylor & Francis; 2010. Chapter 8. Available from: https://www.ncbi.nlm.nih.gov/books/NBK56024/
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